Datasheet І Order Information І Related Products
Description
REzol™ C & T is a ready-to-use reagent for the isolation of total RNA from cells and tissue. The REzol™ C & T RNA isolation method for total RNA is a new improved single-step method and modified from acid guanidine-thiocyanate-phenol-chloroform extract procedure of Chomczynski and Sacchi. The principles for RNA isolation by REzol™ C & T are cell disruption, RNase inactivation and deproteinization of the RNA, and physical separation of the RNA from other macro-molecules. At the acid pH in REzol™ C & T RNA isolation, the solubility of DNA and protein are decreased and partition to the organic phase and interphase, whereas the RNA remains exclusively in the aqueous phase. The total RNA is precipitated from the aqueous phase by addition of isopropanol, wash with ethanol and solubilized in DEPC-treated water.
Feature
• Easy-to-use
• Low DNA and protein contamination
Application
Total RNA purification
Storage Condition
4 °C
Order Information
REzol C&T
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Catalog NO.
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Package
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Price US$
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PT-KP200CT
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200 ml
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