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  SuperRun 8-Strip RT-PCR Mix  
 

                                                                                                                                                                  

 

Datasheet І Order Information І Related Products

 

Description

The SuperRun 8-Strip RT-PCR Mix is a ready to use RT-PCR mixture of specific target gene from either total RNA or mRNA. The mix simplifies the procedure and reduces the potential for sample contamination. Therefore, this mix offers a convenient and reproducible analysis of RNAs in one tube. It is supplied as 8-strip -20°C stored package containing lyophilized mixture of MMLV reverse transcriptase and HotStartTaq DNA polymerase, tracking dyes and all the other components needed for RT-PCR reaction. MMLV reverse transcriptase has weak RNase H activity compared to AMV reverse transcriptase and made it a better RT enzyme for full-length cDNA synthesis. Users only have to add primers, template, and water to a final volume of 20 μl to have a complete RT-PCR reaction mix. The amplified DNA fragments will generate a 3’ A-overhang that can be cloned by TA or GC cloning. After RT-PCR program finished, the presence of premixed dyes, red (~600 bp on 2% agarose gel) and yellow (~50 bp on 2% agarose gel), allows users to directly load the reaction mix onto the agarose gel for DNA electrophoresis.

 

Feature

• 8-strip format

• Ready-to-use RT-PCR master mix

• MMLV reverse transcriptase

• Directly gel loading

• Lyophilized

 

Application

mRNA quantitation

cDNA cloning

One step RT-PCR

 

Storage Condition

-20 °C

 

Order Information

SuperRun 8-Strip RT-PCR Kit

Catalog NO.

Package

Price US$

PT-8RP100

8-strip x 12

 

Related Poducts

Fast-Run HotStart RT-PCR Mix

SuperRun RT-PCR Mix

REzol C&T

RNase/DNase Terminator

AGAROSE

 
     
 

 

 
Protech Technology Enterprise CO., Ltd.
14F-C,NO.3, (BuildingF), Yuan-Qu St, Nanking Dist, Taipei, Taiwan (R.O.C.)
TEL: 886-2-26557677    E-mail: international@bio-protech.com.tw